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Proceedings Paper

New portable time-resolved photometer for monitoring the calcium dynamics of osteoblasts under mechanical and zero-gravity stimulation
Author(s): Jens Struckmeier; Jochen Tenbosch; Erk Klopp; Matthias Born; Martin R. Hofmann; David B. Jones
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Paper Abstract

We introduce a compact and portable photometric system for measurements of the calcium dynamics in cells. The photometer is designed for applications in centrifuges or in zero-gravity environment and thus extremely compact and reliable. It operates with the calcium-sensitive dye Indo-1. The excitation wavelength of 345nm is generated by frequency doubling of a laser diode. Two compact photomultiplier tubes detect the fluorescent emission. The electronics provides the sensitivity of photon counting combined with simultaneous measurement of the temperature, of air pressure, and of gravitational force. Internal data storage during the experiment is possible. A newly developed cell chamber stabilizes the cell temperature to 37.0 percent C +/- 0.1 degree C and includes a perfusion system to supply the cells with medium. The system has a modular set-up providing the possibility to change light source and detectors for investigation of other ions than calcium. Quantitative measurements of the intracellular calcium concentration are based on a comprehensive calibration of our system. First experiments show that the calcium dynamics of osteosarcoma cells stimulated by parathyroid hormone is observable.

Paper Details

Date Published: 27 April 2000
PDF: 10 pages
Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); doi: 10.1117/12.384203
Show Author Affiliations
Jens Struckmeier, Philipps Univ. Marburg (United States)
Jochen Tenbosch, Philipps Univ. Marburg (Germany)
Erk Klopp, Philipps Univ. Marburg (Germany)
Matthias Born, Philipps Univ. Marburg (Germany)
Martin R. Hofmann, Philipps Univ. Marburg (Germany)
David B. Jones, Philipps Univ. Marburg (Germany)

Published in SPIE Proceedings Vol. 3921:
Optical Diagnostics of Living Cells III
Daniel L. Farkas; Daniel L. Farkas; Robert C. Leif, Editor(s)

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