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Proceedings Paper

Confocal imaging through scattering media with a volume holographic filter
Author(s): Michal Balberg; George Barbastathis; Sergio Fantini; David J. Brady
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Paper Abstract

The use of a volume holographic filter as collector element in a confocal system imaging through scattering (turbid) material is described. We show that the penetration depth of the volume holographic system is de-coupled from the scatter noise discrimination properties, and is potentially more advantageous than the traditional confocal microscope. Since the volume-holographic filter is a matched-filter, the penetration depth is dependent on the mismatch of the refractive index of the sample being imaged relative to the recording conditions. We present a method to overcome this limitation based on using a pre-compensating index matching film during the recording of the volume hologram. An improvement of the penetration depth is shown experimentally.

Paper Details

Date Published: 2 May 2000
PDF: 6 pages
Proc. SPIE 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII, (2 May 2000); doi: 10.1117/12.384180
Show Author Affiliations
Michal Balberg, Univ. of Illinois/Urbana-Champaign (United States)
George Barbastathis, Massachusetts Institute of Technology (United States)
Sergio Fantini, Tufts Univ. (United States)
David J. Brady, Univ. of Illinois/Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 3919:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII
Jose-Angel Conchello; Carol J. Cogswell; Andrew G. Tescher; Tony Wilson, Editor(s)

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