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Proceedings Paper

Direct observation of supramolecular structures of biorelated materials by atomic force microscopy
Author(s): Hiroyasu Yamaguchi; Kei Kubota; Akira Harada
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Paper Abstract

We have prepared DNA catenanes and studied the topological structures of DNA catenanes by atomic force microscopy (AFM) and electrophoresis. Nicked DNA was synthesized by the addition of DNASE I to a solution of plasmid pBR322. Catenated DNA molecules were prepared by the reaction of topoisomerase I with nicked DNA. Catenation reactions were monitored by the agarose gel electrophoresis. A droplet of solution, containing DNA catenanes extracted from the band of 1.0 percent agarose gel electrophoresis by a centrifugal filter device, was applied to a freshly cleaved mica surface. After drying the specimens, AFM measurements were carried out by using a silicon cantilever. The single molecule images of DNA catenanes were clearly observed for the first time by AFM using a tapping mode at room temperature and in an ambient atmosphere.

Paper Details

Date Published: 21 April 2000
PDF: 8 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383353
Show Author Affiliations
Hiroyasu Yamaguchi, Osaka Univ. (Japan)
Kei Kubota, Osaka Univ. (Japan)
Akira Harada, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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