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Proceedings Paper

Nanoimaging and ultra structure of Entamoeba histolytica and its pseudopods by using atomic force microscope
Author(s): Narahari V. Joshi; Honorio Medina; H. Urdaneta; J. Barboza
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Paper Abstract

Nan-imaging of Entamoeba histolytica was carried out by using Atomic Force Microscope (AFM). The structure of the nucleus, endoplasm and ectoplasm were studied separately. The diameter of the nucleus in living E. histolytica was found to be of the order of 10 micrometers which is slightly higher than the earlier reported value. The presence of karysome was detected in the nucleus. Well-organized patterns of chromatoid bodies located within the endoplasm, were detected and their repetitive patterns were examined. The organized structure was also extended within the ectoplasm. The dimensions and form of the organization suggest that chromatic bodies are constituted with ribosomes ordered in the form of folded sheet. Such structures were found to be absent in non-living E. histolytica. AFM images were also captured just in the act when ameba was extending its pseudopods. Alteration in the ultrastructure caused during the process of extension was viewed. Well marked canals of width 694.05 nm. And height 211.05 nm are clearly perceptible towards the direction of the pseudopods. 3D images are presented to appreciate the height variation, which can not be achieved by conventional well-established techniques such as electron microscopy.

Paper Details

Date Published: 21 April 2000
PDF: 6 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383352
Show Author Affiliations
Narahari V. Joshi, Univ. de Los Andes (Spain)
Honorio Medina, Univ. de Los Andes (Venezuela)
H. Urdaneta, Univ. de Los Andes (Venezuela)
J. Barboza, Univ. de Los Andes (Venezuela)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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