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Proceedings Paper

Fluorescence apertureless near-field microscope: a step toward imaging information in DNA
Author(s): Stephen R. Quake; T. Jason Yang; Guillaume A. Lessard; Marc Unger; Emil Kartalov
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Paper Abstract

Single molecule imaging with optical methods has become an important tool in biophysical studies. However, when imaging molecules at room temperature using far field optics, one can only resolve molecules that are separated by a distance greater than the diffraction limit of the microscope, about 220 nanometers. Near field techniques have allowed researchers to image with resolutions on the order of 30-50 nanometers. However, there are numerous reasons to try to push the resolution limit further. One that particular concerns our group is the \notion to try to image information in DNA in order to measure sequence information. To that end, we have developed a new type of near field microscope, the fluorescence apertureless near field microscope.

Paper Details

Date Published: 21 April 2000
PDF: 6 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383349
Show Author Affiliations
Stephen R. Quake, California Institute of Technology (United States)
T. Jason Yang, California Institute of Technology (United States)
Guillaume A. Lessard, California Institute of Technology (United States)
Marc Unger, California Institute of Technology (United States)
Emil Kartalov, California Institute of Technology (United States)


Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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