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Proceedings Paper

Near-field imaging of neurotransmitter release and uptake in patterned neuron networks
Author(s): Patrick Degenaar; Yuji Murakami; Kenji Yokoyama; Eiichi Tamiya; Bruno Le Pioufle; Yiroyuki Fujita
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Paper Abstract

A SNOAM system is capable of obtaining simultaneous topographic and optical images with a resolution beyond than the diffraction limit of far field optical imaging. Fluorescence tagging combined with optical resolutions of better than 100nm allow us to detect structures not possible with conventional microscopes. Also in contrast with electron microscopy SNOAM has the ability to look at biological structures in the liquid medium. Presently there is much interest in understanding the processes that lead to LTP in neuron synapses. LTP is widely associated with memory function in neurons. Hence, better understanding will lead to advances in medicine, as well as neuron-based memory and processing devices. Better understanding is also crucial to the development of neuron-electronic interfaces. In this research, neuron networks are grown on a patterned polylysine substrate. Polylysine is patterned using micro lithographic techniques. Neurons are extracted from the hippocampus of chick embryos, and are then grown on this pattern under standard sterile incubating conditions. The neurons are stimulated to release the neurotransmitter glutamate. The glutamate is then fluorescently imaged with Amplex-red SNOAM.

Paper Details

Date Published: 21 April 2000
PDF: 10 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383348
Show Author Affiliations
Patrick Degenaar, Japan Advanced Institute of Science and Technology (Japan)
Yuji Murakami, Japan Advanced Institute of Science and Technology (Japan)
Kenji Yokoyama, Japan Advanced Institute of Science and Technology (Japan)
Eiichi Tamiya, Japan Advanced Institute of Science and Technology (Japan)
Bruno Le Pioufle, Univ. of Tokyo (Japan)
Yiroyuki Fujita, Univ. of Tokyo (Japan)


Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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