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Proceedings Paper

Synthesis, characterization, and application of fluorescent lipobeads for imaging and sensing in single cells
Author(s): Kerry P. McNamara; Nitsa Rosenzweig; Zeev Rosenzweig
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Paper Abstract

This paper describes the synthesis and characterization of submicron phospholipid coated polystyrene particles, named lipobeads, with pH sensing capability. The phospholipids used to coat the particles are labeled with fluorescein and tetramethylrhodamine, which serves as a referencing fluorophore for increased accuracy of the pH measurements. The synthesis of the pH sensing lipobeads is realized by the covalent attachment of the fluorescent phospholipids to the surface of styrene-divinylbenzene micron or submicron sized particles. The pH dynamic range of the sensing particles is between pH 5.5 and 7 with a sensitivity of 0.1 pH units and they are photostable under the experimental conditions used in these studies. The fluorescent lipobeads are used to monitor pH changes in volume limited samples and to measure the pH of single macrophages. The lipobeads are ingested by the macrophages and directed to lysosomes, which are the cellular organelles involved in the phagocytosis process. Despite the high lyososomal levels of digestive enzymes and acidity, the absorbed particles remain stable for 6 hours in the cells when the cells are stored in a PBS buffer solution at pH 7.4.

Paper Details

Date Published: 21 April 2000
PDF: 11 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383342
Show Author Affiliations
Kerry P. McNamara, Univ. of New Orleans (United States)
Nitsa Rosenzweig, Xavier Univ. of Louisiana (United States)
Zeev Rosenzweig, Univ. of New Orleans (United States)


Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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