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Proceedings Paper

Novel optical biosensors using a gold colloid monolayer substrate
Author(s): Heather A. Clark; Greg Merritt; Raoul Kopelman
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Paper Abstract

A novel optical biosensor matrix has been developed to exploit the native fluorescence of certain proteins. This matrix uses a gold colloid monolayer attached to an end of a fiber as a substrate for protein attachment. The effect of the gold monolayer size has been investigated through the techniques of fluorescence, scanning electron microscopy, and transmission electron microscopy. It has been shown that the size of the gold colloid does produce a marked difference in the fluorescence intensity measured. It is surmised through the use of microscopy techniques that the intensity changes seen in the fluorescence emission are not a result of surface coverage, or availability of sites for protein adsorption, but instead of quenching or enhancement by the gold itself.

Paper Details

Date Published: 21 April 2000
PDF: 9 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383341
Show Author Affiliations
Heather A. Clark, Univ. of Michigan (United States)
Greg Merritt, Univ. of Michigan (United States)
Raoul Kopelman, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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