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Proceedings Paper

Single molecule detection in single living cells
Author(s): Tyler A. Byassee; Warren C. W. Chan; Shuming Nie
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Paper Abstract

The ability to detect a single analyte molecule represents the ultimate in sensitivity. Single molecule detection has emerged as a powerful tool to characterize heterogeneous systems, in which traditional bulk sampling methods provide a signal averaged over a large number of analytes. Traditionally, single molecule measurements have required highly controlled experimental conditions using ultrapure solvents to create a minimum level of interference. These constraints have primarily limited this technique to examination of systems in vitro. In this report we present the first instance of real-time single molecule detection in living cells. Our experimental approach allows dynamic monitoring of individual fluorophores in vivo, despite the highly complex cellular environment.

Paper Details

Date Published: 21 April 2000
PDF: 9 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383337
Show Author Affiliations
Tyler A. Byassee, Indiana Univ. (United States)
Warren C. W. Chan, Indiana Univ. (United States)
Shuming Nie, Indiana Univ. (United States)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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