Share Email Print
cover

Proceedings Paper

Single-molecule adsorption at nanometer indentations
Author(s): Mary Jean Wirth; Derrick J. Swinton; Melody D. Ludes; Leon J. Doneski; Cozette M. Cuppett; Hui Zhang
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The temporal behavior of bursts from single molecules reveals whether adsorbates are diffusing at chemical interfaces or are specifically adsorbed. The chemical interface of water/C18-monolayer on silica is studied. Burst data for single molecules of DiI show that regions of the surface having more nanoindentations are associated with a greater number of specific adsorption events. Lysozyme is shown to adsorb irreversibly, and an oligonucleotide is shown to adsorb reversibly but more strongly than DiI to nanointendations at this interface. For each adsorbate, the specific adsorption at the nanoindentations is attributed to hydrogen bonding to exposed silanols.

Paper Details

Date Published: 21 April 2000
PDF: 8 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383336
Show Author Affiliations
Mary Jean Wirth, Univ. of Delaware (United States)
Derrick J. Swinton, Univ. of Delaware (United States)
Melody D. Ludes, Univ. of Delaware (United States)
Leon J. Doneski, Univ. of Delaware (United States)
Cozette M. Cuppett, Univ. of Delaware (United States)
Hui Zhang, Univ. of Delaware (United States)


Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

© SPIE. Terms of Use
Back to Top