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Proceedings Paper

Imaging of a single DNA molecule by AFM: study of DNA conformation change in HMG-DNA complex
Author(s): Hiroshi Muramatsu; Jong-Min Kim; Toshio Otani; Katsunori Homma; Mitsuteru Yoshida; Shin-ichi Tate; Masato Saito; Eiichi Tamiya
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Paper Abstract

Conformation change of DNA under binding a DNA binding protein was studied using an AFM. An originally designed AFM mounted on an inverse microscope was used for imaging DNA. High mobility group (HMG) protein and pUC118 DNA were used as DNA binding protein and DNA for investigation in this study. The pUC118 DNA and its mixture with HMG2BJ were imaged by the AFM. In the AFM image of pUC118, DNA strands showed open structure with a few helix sites. The height of the helix part is 1.5 times higher than the ordinary DNA strand. Centrally AFM images of the mixture showed HMG-like particles on DNA strands. The height of the particle is 2-3 times higher than the DNA strands which showed the particles were not aggregating parts but HMG. The HMG bound on a crossing part of DNA which make a loop.

Paper Details

Date Published: 21 April 2000
PDF: 6 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383335
Show Author Affiliations
Hiroshi Muramatsu, Seiko Instruments Inc. (Japan)
Jong-Min Kim, National Food Research Institute (Japan)
Toshio Otani, National Food Research Institute (Japan)
Katsunori Homma, Seiko Instruments Inc. (Japan)
Mitsuteru Yoshida, Science Univ. of Tokyo (Japan)
Shin-ichi Tate, Japan Advanced Institute of Science and Technology (Japan)
Masato Saito, Japan Advanced Institute of Science and Technology (Japan)
Eiichi Tamiya, Japan Advanced Institute of Science and Technology (Japan)


Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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