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Proceedings Paper

Metabolic cytometry
Author(s): Sergey N. Krylov; Zheru Zhang; Norman J. Dovichi
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Paper Abstract

Capillary electrophoresis with laser-induced fluorescence detection has the potential to become an important tool in analyzing the chemical contents of single cells. Singe-cell studies are important for understanding the molecular mechanisms of many biological processes, including serious disorders, such as concern and neurodegradative diseases. We developed a new technique, Metabolic Cytometry, that allows us to study complex metabolic pathways in single cells. The essence of the technique is the following. A fluorescently labeled metabolic probe is introduce by incubation into a cell where it is converted to a number of fluorescently labeled metabolites. After incubation the cell is injected into the capillary and lysed. The metabolites are then separated and detected using CE-LIF. If metabolic cytometry is combined with image cytometry, then metabolic activities can be correlated with cellular phenotype or genotype. We applied this powerful approach to correlate glycosylation with the cell's phase in the cell cycle.

Paper Details

Date Published: 21 April 2000
PDF: 8 pages
Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383332
Show Author Affiliations
Sergey N. Krylov, Univ. of Alberta (Canada)
Zheru Zhang, Univ. of Alberta (Canada)
Norman J. Dovichi, Univ. of Alberta (United States)

Published in SPIE Proceedings Vol. 3922:
Scanning and Force Microscopies for Biomedical Applications II
Shuming Nie; Eiichi Tamiya; Edward S. Yeung, Editor(s)

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