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Proceedings Paper

Light-scattering microscope as a tool to investigate scattering heterogeneity in tissue
Author(s): Alois K. Popp; Megan T. Valentine; Peter D. Kaplan; David A. Weitz
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Paper Abstract

Rayleigh light scattering has not yet been used for quantitative investigations of heterogeneous systems. Preconditions such an experiment are a well defined scattering geometry and independent information about the local state of the sample. We have designed a new instrument that meets these criteria: a light-scattering microscope with simultaneous imaging. We demonstrate the ability to characterize local differences within one tissue type as well as global differences between tissue types. Real space images of the sample are taken by normal video microscopy techniques. The light scattering pattern in analyzed by the evaluation of wave-vector dependence and scattering direction of the scattered intensity. Statistical analysis of scattering patterns show what is important for the characterization and classification of tissues and heterogeneous structures. Real space images provide context for scattering analysis. The light scattering microscope is a powerful tool for characterization of local structural order in inhomogeneous structures like tissues.

Paper Details

Date Published: 19 April 2000
PDF: 11 pages
Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); doi: 10.1117/12.382746
Show Author Affiliations
Alois K. Popp, Harvard Univ. (United States)
Megan T. Valentine, Harvard Univ. (United States)
Peter D. Kaplan, Unilever Research U.S. (United States)
David A. Weitz, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 3917:
Optical Biopsy III
Robert R. Alfano, Editor(s)

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