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Proceedings Paper

Enhancement of ultrasound reflectivity depends on the specific perfluorocarbons utilized to formulate nanoparticle emulsion contrast agents
Author(s): Jon N. Marsh; Christopher S. Hall; Michael J. Scott; Ralph J. Fuhrhop; Patrick J. Gaffney; Samuel A. Wickline; Gregory M. Lanza
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Paper Abstract

A nongaseous, ligand-targeted perfluorocarbon nanoparticle emulsion has been developed which can acoustically enhance the presence of molecular epitopes on tissue surfaces. We demonstrate the impact of incorporating perfluorocarbons with specific phase velocities into the emulsions on the acoustic reflectivity of plasma clots targeted using these nanoparticles. Porcine plasma clots were targeted in vitro with specific perfluorocarbon emulsions using anti-fibrin antibody solution (NIB 5F3). Five perfluorocarbons were investigated: perfluorohexane, perfluorooctyl-bromide, perfluorooctane, perfluorodichlorooctane, and perfluorodecalin. Ultrasonic backscatter (17 - 35 MHz) was measured at the front surface of the clots. Backscatter enhancement was determined by comparison with untreated clots. The magnitude of enhancement depended on the perfluorocarbon emulsion used. Perfluorohexane and perfluorooctane exhibited the greatest enhancement relative to untreated clots (23 dB) and perfluorodecalin the least (18 dB), consistent with predictions from a simple acoustic transmission-line model. We conclude that targeted, nongaseous perfluorocarbon contrast agents can significantly increase the sensitivity of ultrasonic detection of low-scattering biological media, and that further optimization of these contrast agents can be realized by judicious choice of the emulsified perfluorocarbon.

Paper Details

Date Published: 12 April 2000
PDF: 8 pages
Proc. SPIE 3982, Medical Imaging 2000: Ultrasonic Imaging and Signal Processing, (12 April 2000); doi: 10.1117/12.382242
Show Author Affiliations
Jon N. Marsh, Washington Univ. School of Medicine (United States)
Christopher S. Hall, Washington Univ. School of Medicine (United States)
Michael J. Scott, Washington Univ. School of Medicine (United States)
Ralph J. Fuhrhop, Washington Univ. School of Medicine (United States)
Patrick J. Gaffney, National Institute for Biological Standards and Control (United Kingdom)
Samuel A. Wickline, Washington Univ. School of Medicine (United States)
Gregory M. Lanza, Washington Univ. School of Medicine (United States)


Published in SPIE Proceedings Vol. 3982:
Medical Imaging 2000: Ultrasonic Imaging and Signal Processing
K. Kirk Shung; Michael F. Insana, Editor(s)

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