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Proceedings Paper

Nucleic acid in-situ hybridization detection of infectious agents
Author(s): Curtis T. Thompson
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Paper Abstract

Limitations of traditional culture methods and newer polymerase chain reaction (PCR)-based methods for detection and speciation of infectious agents demonstrate the need for more rapid and better diagnostics. Nucleic acid hybridization is a detection technology that has gained wide acceptance in cancer and prenatal cytogenetics. Using a modification of the nucleic acid hybridization technique known as fluorescence in-situ hybridization, infectious agents can be detected in a variety of specimens with high sensitivity and specificity. The specimens derive from all types of human and animal sources including body fluids, tissue aspirates and biopsy material. Nucleic acid hybridization can be performed in less than one hour. The result can be interpreted either using traditional fluorescence microscopy or automated platforms such as micro arrays. This paper demonstrates proof of concept for nucleic acid hybridization detection of different infectious agents. Interpretation within a cytologic and histologic context is possible with fluorescence microscopic analysis, thereby providing confirmatory evidence of hybridization. With careful probe selection, nucleic acid hybridization promises to be a highly sensitive and specific practical diagnostic alternative to culture, traditional staining methods, immunohistochemistry and complicated nucleic acid amplification tests.

Paper Details

Date Published: 11 April 2000
PDF: 6 pages
Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); doi: 10.1117/12.382042
Show Author Affiliations
Curtis T. Thompson, Univ. of New Mexico Health Sciences Ctr. and Sunfish Detection Technologies, Inc. (United States)

Published in SPIE Proceedings Vol. 3913:
In-Vitro Diagnostic Instrumentation
Gerald E. Cohn, Editor(s)

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