Share Email Print
cover

Proceedings Paper

Issues in enzyme-based metal ion biosensing in complex media
Author(s): Richard B. Thompson; Hui-Hui Zeng; Michele Loetz; Carol A. Fierke
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In developing fluorescence-based biosensors for rapidly determining metal ions such as zinc or copper in complex media such as sea water, serum, cerebrospinal fluid, or the interior of a cell, several issues must be considered. Among these are the selectivity, sensitivity, ease of calibration, speed of response, reversibility, stability, and the ease of immobilization onto a solid substrate. While the first three have been dealt with in the design of our transducer molecule, apocarbonic anhydrase II, the others remain to be considered. In this paper we examine the stability of the apoprotein to storage at various temperatures and pHs with a view to establishing storage conditions and lifetimes in operating environments for sensor transducers. Similarly, we immobilized a storage conditions and lifetimes in operating environments for sensor transducers. Similarly, we immobilized a fluorescent-labeled apo-CA variant on quartz to determine its sped, sensitivity, and kinetics of response.

Paper Details

Date Published: 11 April 2000
PDF: 8 pages
Proc. SPIE 3913, In-Vitro Diagnostic Instrumentation, (11 April 2000); doi: 10.1117/12.382025
Show Author Affiliations
Richard B. Thompson, Univ. of Maryland/Baltimore School of Medicine (United States)
Hui-Hui Zeng, Univ. of Maryland/Baltimore School of Medicine (United States)
Michele Loetz, Univ. of Maryland/Baltimore School of Medicine (United States)
Carol A. Fierke, Univ. of Michigan (United States)


Published in SPIE Proceedings Vol. 3913:
In-Vitro Diagnostic Instrumentation
Gerald E. Cohn, Editor(s)

© SPIE. Terms of Use
Back to Top