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Proceedings Paper

QLF monitoring of therapies for early secondary caries arrestment and remineralization
Author(s): Margherita Fontana; Carlos Gonzalez-Cabezas; George K. Stookey
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Paper Abstract

Secondary caries (SC) is the most common reason for restoration failure. The purpose of this study was to evaluate the Quantitative Light-Induced Fluorescence (QLF) method for monitoring therapies to inhibit SC progression. Forty-eight human teeth with resin restorations were demineralized for 4 days in a microbial caries model. Half of each specimen was then covered with an acid-resistant varnish to maintain the baseline lesion, and treated (group 1: non-treated control; group 2: chlorhexidine varnish for 24 h; group 3: fluoride varnish for 24 h; group 4: APF topical fluoride gel for 4 min), prior to being demineralized for 4 more days. Specimens were analyzed by QLF, sectioned, stained with Rhodamine B, and analyzed with a confocal microscope (CLSM) for lesion depth. The QLF results indicated that the control group was significantly (p less than 0.05) different (i.e., lesions progressed) from groups treated with fluoride (groups 3 and 4; lesions remineralized). All other group comparisons were not significantly different. Results obtained from CLSM analysis were similar to the ones obtained with QLF, except that lesions in group 2 were significantly deeper than the ones in the fluoride groups. Results suggest that the QLF method has a clear potential for monitoring remineralizing therapies for SC.

Paper Details

Date Published: 24 March 2000
PDF: 8 pages
Proc. SPIE 3910, Lasers in Dentistry VI, (24 March 2000); doi: 10.1117/12.380845
Show Author Affiliations
Margherita Fontana, Indiana Univ. School of Dentistry (United States)
Carlos Gonzalez-Cabezas, Indiana Univ. School of Dentistry (United States)
George K. Stookey, Indiana Univ. School of Dentistry (United States)


Published in SPIE Proceedings Vol. 3910:
Lasers in Dentistry VI
John D. B. Featherstone; Peter Rechmann; Daniel Fried, Editor(s)

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