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Proceedings Paper

Effect of diode laser radiation in root canal wall dentine: a microbiological study
Author(s): Norbert Gutknecht; Georg Conrads; Christian Apel; Claus Schubert; Friedrich Lampert
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Paper Abstract

The aim of this study was to investigate the antibacterial effect of a diode laser in deep root canal dentine. The microbial colonization of root canal dentine can lead to failures in conventional endodontic treatment if only an inadequate bacterial reduction is achieved through canal treatment and chemical disinfection. 100 micrometer, 300 micrometer and 500 micrometer bovine dentine slices obtained by longitudinal sections were sterilized and inoculated on one side with an Enterococcus faecalis suspension. Laser radiation was performed on the opposite side with the diode laser, emits light at 810 nm and operates in the continuous wave mode (cw). Radiation was performed using a 400 micrometer tapered fiber tip at an angle of approx. 5 degrees to the surface over a period of 30 s. The output power at the distal end of the tip was 0.6 watt. The bacteria were then eluted through vibration and cultured on blood agar plates. The colony count reflected the antibacterial effect of laser radiation as a function of the layer thickness. A mean bacterial reduction of 74% was achieved even with a 500 micrometer thick slice. This investigation indicates that the diode laser can support the bacterial reduction in endodontic treatment.

Paper Details

Date Published: 24 March 2000
PDF: 4 pages
Proc. SPIE 3910, Lasers in Dentistry VI, (24 March 2000); doi: 10.1117/12.380819
Show Author Affiliations
Norbert Gutknecht, Rheinisch-Westfaelische Technische Hochschule Aachen (Germany)
Georg Conrads, Rheinisch-Westfaelische Technische Hochschule Aachen (Germany)
Christian Apel, Rheinisch-Westfaelische Technische Hochschule Aachen (Germany)
Claus Schubert, Rheinisch-Westfaelische Technische Hochschule Aachen (Germany)
Friedrich Lampert, Rheinisch-Westfaelische Technische Hochschule Aachen (Germany)

Published in SPIE Proceedings Vol. 3910:
Lasers in Dentistry VI
John D. B. Featherstone; Peter Rechmann; Daniel Fried, Editor(s)

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