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Proceedings Paper

Effects of LLLT on the proliferation of HEp2 cells: study in vitro
Author(s): Antonio Luiz Barbosa Pinheiro; Silene Carneiro Nascimento; Alessandro L. B. Vieira; Aluizio Barros Rolim; Pedro Soriano da Silva; Aldo Brugnera
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Paper Abstract

LLLT has been used successfully in biomedicine and some of the results are thought to be related to cell proliferation. The effects of LLLT on cell proliferation is debatable because different studies found either increase or decrease on proliferation of cell cultures. Cell culture is an excellent method to assess both effects and dose of treatment. The aim of this study was to assess the effect off 635 and 670 nm laser irradiation of H.Ep.2 cells In Vitro using MTT. The cells were obtained from SCC of the larynx were routinely processed from defrost to the experimental condition. Twenty four hours after transplantation the cells were irradiated with doses ranging from 0.03 a 0.6 J/cm2 during seven days. The results showed that 635 nm laser light does not stimulate significantly the proliferation of H.Ep.2 cells in doses ranging from 0.04J/cm2 to 0.488J/cm2. However 670 nm laser irradiation resulted in an increased cell proliferation when compared to both control and 635 nm irradiated cells. It is concluded that irradiation of H.Ep.2 cells with 670 nm laser results in an increased cell proliferation; best observation of cell proliferation was found on 670 nm laser irradiated cultures exposed from doses of 0.075 J/cm2; both dose and wavelengths are factors which may affect cell proliferation of H.Ep.2 cells; doses of 635 nm laser light up to 0.48J/cm2 did not interfere with H.Ep.2 cell proliferation; and that the MTT was effective on the detection of H.Ep.2 cells proliferation on both irradiated and non-irradiated cell cultures.

Paper Details

Date Published: 24 March 2000
PDF: 7 pages
Proc. SPIE 3910, Lasers in Dentistry VI, (24 March 2000); doi: 10.1117/12.380810
Show Author Affiliations
Antonio Luiz Barbosa Pinheiro, Univ. Federal da Bahia (Brazil)
Silene Carneiro Nascimento, Univ. Federal de Pernambuco (Brazil)
Alessandro L. B. Vieira, Univ. Federal da Bahia (Brazil)
Aluizio Barros Rolim, Univ. Federal de Pernambuco (Brazil)
Pedro Soriano da Silva, Univ. Federal de Pernambuco (Brazil)
Aldo Brugnera, Univ. do Vale do Paraiba (Brazil)


Published in SPIE Proceedings Vol. 3910:
Lasers in Dentistry VI
John D. B. Featherstone; Peter Rechmann; Daniel Fried, Editor(s)

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