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Proceedings Paper

Process of thermal destruction of glucose in consequence of sterilization in autoclave in the biopharmaceutical manufacturing
Author(s): Volodymyr M. Musyanovych; Olexander I. Bilyi; Nataliia I. Gudz; Andrii V. Zdyrko
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Paper Abstract

The results of studying the process of glucose' thermal decomposition in consequence of the sterilization in the autoclave and the shelf life are considered. The research of the process was carried out for 5% glucose solution for injections before and after the sterilization in the autoclave by saturated steam at 110 degree(s)C for 30 minutes by the study of UV absorption spectra, granulometric analysis in the interval 0.2 - 25.0 mkm and pH measurements. The spectra of the glucose solutions before the sterilization were found to have no absorption bands in the range from 200 nm to 400 nm. After the sterilization two absorption bands with maximal at 229 nm and 284 nm respectively appeared. After three-month storage only one absorption band with maximum at 284 nm remains. In the report the observed spectral effects are explained.

Paper Details

Date Published: 22 March 2000
PDF: 3 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380516
Show Author Affiliations
Volodymyr M. Musyanovych, State Enterprise Lvivdialic (Ukraine)
Olexander I. Bilyi, The Ivan Franko National Univ. of Lviv (Ukraine)
Nataliia I. Gudz, State Enterprise Lvivdialic (Ukraine)
Andrii V. Zdyrko, State Enterprise Lvivdialic (Ukraine)

Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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