Share Email Print
cover

Proceedings Paper

Choline oxidase: a useful tool for high-throughput assays of acetylcholinesterase, phospholipase D, phosphatidylcholine-specific phospholipase C, and sphingomyelinase
Author(s): Mingjie Zhou; Cailan Zhang; Richard P. Haugland
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Choline oxidase converts one molecule of choline to generate two molecules of H2O2. In a 1:1 reaction stoichiometry, horseradish peroxidase-catalyzed oxidation of the nonfluorescent and colorless H2O2 indicator, the Amplex Red reagent, by H2O2 produces the highly fluorescent resorufin, which has an excitation maximum at 563 nm and emission maximum at 587 nm. Using the Amplex Red reagent as an H2O2 indicator, assays of phospholipase D, phosphatidylcholine-specific phospholipase C, acetylcholine, acetylcholinesterase, and sphingomyelinase have been developed in choline oxidase- and HRP-coupled reactions. These assays have been developed in a microplate with a one-step procedure.

Paper Details

Date Published: 22 March 2000
PDF: 6 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380507
Show Author Affiliations
Mingjie Zhou, Molecular Probes, Inc. (United States)
Cailan Zhang, Molecular Probes, Inc. (United States)
Richard P. Haugland, Molecular Probes, Inc. (United States)


Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

© SPIE. Terms of Use
Back to Top