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Proceedings Paper

Use of MagneSil(TM) paramagnetic particles for plasmid purification, PCR cleanup, and purification of dideoxy and big dye DNA sequencing reactions
Author(s): Rex M. Bitner; Doug White; Steven K. Krueger; Michael Bjerke; Braeden Butler; Craig Smith
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Paper Abstract

Traditional anion exchange purification of nucleic acids requires the elution of the DNA or RNA in a salt solution, necessitating the precipitation or desalting of the nucleic acid prior to many molecular biology applications. A pH dependent anion exchange purification method is described which allows the purification of nucleic acids at one pH, followed by the elution of the nucleic acid in a low salt buffer at a second, higher pH. The benefits of this method include the avoidance of alcohol washes and the drying steps required for alcohol removal, as well as the benefits of anion exchange purification without the need for desalting of the purified DNA or RNA.

Paper Details

Date Published: 22 March 2000
PDF: 8 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380504
Show Author Affiliations
Rex M. Bitner, Promega Corp. (United States)
Doug White, Promega Corp. (United States)
Steven K. Krueger, Promega Corp. (United States)
Michael Bjerke, Promega Corp. (United States)
Braeden Butler, Promega Corp. (United States)
Craig Smith, Promega Corp. (United States)


Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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