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Proceedings Paper

Reversed enzyme activity DNA interrogation test: a new method for mutation detection
Author(s): Michelle Mandrekar; Ken Lewis; Donna Leippe; Ryan Olson; John W. Shultz
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Paper Abstract

The Reversed Enzyme Activity DNA Interrogation Test (READITTM) System provides a new, rapid method for genotype determination. The system can be used for the analysis of single nucleotide polymorphisms as well as insertions, deletions and chromosomal rearrangements. The READITTM System is based upon driving various enzymatic reactions in the reverse direction from the direction they are normally considered. The method is based on a pyrophosphorylation reaction that is the reverse of DNA polymerization. Interrogation probes annealed to DNA fragments with which they have a 3- mismatch are not a substrate for the reaction and do not generate significant signals compared with probes that match the interrogation sequence perfectly. By interrogation of specific DNA sequences with carefully designed probes, mutant genes can be identified with speed and precision.

Paper Details

Date Published: 22 March 2000
PDF: 6 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380502
Show Author Affiliations
Michelle Mandrekar, Promega Corp. (United States)
Ken Lewis, Promega Corp. (United States)
Donna Leippe, Promega Corp. (United States)
Ryan Olson, Promega Corp. (United States)
John W. Shultz, Promega Corp. (United States)

Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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