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Proceedings Paper

High-density oligonucleotide probe arrays
Author(s): Glenn H. McGall; Jacqueline A. Fidenza
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Paper Abstract

High-density DNA probe arrays offer a massively parallel approach to nucleic acid sequence analysis that will have a major impact on gene-based biomedical research and clinical diagnostics. Light-directed synthesis has enabled the large- scale manufacture of arrays containing hundreds of thousands of oligonucleotide probe sequences on a glass `chip' about 1.6 cm2 in size. This method is used to produce high- density GeneChipTM probe arrays, which are now finding widespread use in the detection and analysis of mutations and polymorphisms (`genotyping'), and in a wide range of gene expression studies. This paper will discuss methods for high-resolution photolithographic array fabrication which integrate solid-phase oligonucleotide synthesis, photochemically removable protecting groups, and lithographic techniques adapted from the microelectronics industry.

Paper Details

Date Published: 22 March 2000
PDF: 5 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380501
Show Author Affiliations
Glenn H. McGall, Affymetrix, Inc. (United States)
Jacqueline A. Fidenza, Affymetrix, Inc. (United States)

Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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