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Proceedings Paper

Molecular beacon biosensors for DNA/RNA analysis
Author(s): Xiaohong Fang; Sheldon Schuster; Xiaojing Liu; Tiffany Correll; Peng Zhang; Ruby Tapec; Swadeshmukul Santra; Monde Qhobosheanne; Jane Hua Lou; Weihong Tan
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Paper Abstract

We have developed a variety of novel DNA biosensors using a new class of oligonucleotide probe, molecular beacon (MB). MB has the fluorescence signal transduction mechanism built within the molecules. It can report the presence of specific nucleic acids with high sensitivity and excellent selectivity. Biotinylated MBs have been designed and synthesized for immobilization onto silica surface through avidin-biotin binding. The effect of the avidin-biotin bridge on the MB hybridization has been studied. Our result shows that using streptavidin has less effect than using avidin in MB hybridization. Two kinds of fiber optical DNA sensors have been prepared and characterized: a fiber optic evanescent wave sensor and a submicrometer optical fiber sensor. The sensors are rapid,stable, highly selective, reproducible and regenerable. They have been applied to detect specific DNA and mRNA sequences and to the study of the DNA hybridization kinetics. Silica nanoparticles have also been used for MB immobilization in order to prepare a large quantity of nanometer sized DNA/RNA biosensors.

Paper Details

Date Published: 22 March 2000
PDF: 7 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380499
Show Author Affiliations
Xiaohong Fang, Univ. of Florida (United States)
Sheldon Schuster, Univ. of Florida (United States)
Xiaojing Liu, Univ. of Florida (United States)
Tiffany Correll, Univ. of Florida (United States)
Peng Zhang, Univ. of Florida (United States)
Ruby Tapec, Univ. of Florida (United States)
Swadeshmukul Santra, Univ. of Florida (United States)
Monde Qhobosheanne, Univ. of Florida (United States)
Jane Hua Lou, Univ. of Florida (United States)
Weihong Tan, Univ. of Florida (United States)

Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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