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Proceedings Paper

Fluorescent labeling of DNA for genetic analysis
Author(s): Steve Menchen; Scott Benson; Krishna Upadhya; Pete Theisen; Joan Hauser; Paul Kenney
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Paper Abstract

Labeling of DNA with fluorescent reporter is generally accomplished by attaching fluorescent dyes to the 5'-end of primers for PCR based applications, including geno-typing and linkage mapping. Requirements of spectral resolution and chemical stability have guided us toward asymmetric fluorescein dye structures whose chemical and physical properties have been fine-tuned through substituent modification. We have developed dyes that are amenable to 5'-prime labeling on solid support as dye-amidites facilitating automated dye-primer synthesis.

Paper Details

Date Published: 22 March 2000
PDF: 7 pages
Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380498
Show Author Affiliations
Steve Menchen, PE Biosystems (United States)
Scott Benson, PE Biosystems (United States)
Krishna Upadhya, PE Biosystems (United States)
Pete Theisen, PE Biosystems (United States)
Joan Hauser, PE Biosystems (United States)
Paul Kenney, PE Biosystems (United States)


Published in SPIE Proceedings Vol. 3926:
Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing
Patrick A. Limbach; John C. Owicki; Ramesh Raghavachari; Weihong Tan, Editor(s)

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