Share Email Print
cover

Proceedings Paper

Comparison of PDT efficiencies for photo-oxidation of substrate (NADPH) using a photosensitizer (Photofrin II)
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Photo-oxidation was studied during PDT in a model system using NADPH as the target substrate and Photofrin II as the photosensitizer. The efficiency of NADPH oxidation per photon absorbed by photosensitizer was determined as a function of substrate concentration. The kinetics of changes in absorbance was measured to assay oxidation of NADPH. The efficiency of oxidation increased with increasing NADPH concentration and approached a stable value of 0.0048. Using typical values for the quantum efficiency of the Photofrin triplet state generation and the efficiency of singlet oxygen production we obtained a value of the efficiency of interaction between singlet oxygen and NADPH. Efficiency of diffusion of singlet oxygen was modeled with molecular diffusion theory using typical values for the singlet oxygen lifetime and the diffusion coefficient in aqueous solutions.

Paper Details

Date Published: 29 March 2000
PDF: 7 pages
Proc. SPIE 3909, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX, (29 March 2000); doi: 10.1117/12.379887
Show Author Affiliations
Paulo R. Bargo, Oregon Medical Laser Ctr. (United States)
Parmeswararn Diagaradjane, Anna Univ. (India)
Steven L. Jacques, Oregon Medical Laser Ctr. (United States)


Published in SPIE Proceedings Vol. 3909:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IX
Thomas J. Dougherty, Editor(s)

© SPIE. Terms of Use
Back to Top