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Proceedings Paper

Cytogenetic analysis of interphase cells using spectral imaging [SIm] technology
Author(s): Heinz-Ulli G. Weier; Jan Smida; Horst Zitzelsberger; Robert A. Lersch; Jamie Hung; H. Ben Hsieh; K. Salassidis; George McNamara; Roger A. Pedersen; Jingly Fung
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Paper Abstract

Spectral Imaging (SIm) has dramatically improved our ability to localize and quantitatively analyze multiple nucleic acid targets such as chromosomes, genes and gene transcripts. Studies on metaphase cells such as `Spectral Karyotyping' are less complicated than interphase cell studies because the objects (chromosomes) are spatially separated and ratio- labeled probes can be used to uniquely stain each chromosome type. Our research, however, targets the extensive cytogenetic and phenotypic analysis of interphase cells. The complex organization of interphase chromatin and co- localization of gene transcripts (RNAs) in nuclear or cytoplasmic domains requires unique fluorochrome-labeling for each nucleic acid target. An increasing number of commercially available dyes for probe labeling and software to deconvolute partially overlapping emission spectra has helped to overcome most of these obstacles. This presentation summarizes our experience in analyzing numerical and structural alterations in various human cell types (leukocytes, amniocytes, blastomeres or solid tissue) as well as our approach to multi-gene expression profiling using SIm. Examples illustrate a wide spectrum of groundbreaking techniques for interphase cell analysis. We demonstrate how ten or more chromosomes can be scored in interphase nuclei or the relative level of expression of different transforming RNAs in tumor cells can be measured by SIm.

Paper Details

Date Published: 14 March 2000
PDF: 10 pages
Proc. SPIE 3920, Spectral Imaging: Instrumentation, Applications, and Analysis, (14 March 2000); doi: 10.1117/12.379585
Show Author Affiliations
Heinz-Ulli G. Weier, Lawrence Berkeley National Lab. (United States)
Jan Smida, Institute of Radiobiology (Germany)
Horst Zitzelsberger, Institute of Radiobiology and Ludwig-Maximilians-Univ. Muenchen (Germany)
Robert A. Lersch, Lawrence Berkeley National Lab. (United States)
Jamie Hung, Lawrence Berkeley National Lab. (United States)
H. Ben Hsieh, Lawrence Berkeley National Lab. (United States)
K. Salassidis, Institute of Radiobiology (Germany)
George McNamara, Applied Spectral Imaging, Inc. (United States)
Roger A. Pedersen, Univ. of California/San Francisco (United States)
Jingly Fung, Lawrence Berkeley National Lab. and Univ. of California/San Francisco (United States)


Published in SPIE Proceedings Vol. 3920:
Spectral Imaging: Instrumentation, Applications, and Analysis
Gregory H. Bearman; Dario Cabib; Richard M. Levenson, Editor(s)

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