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Proceedings Paper

Laser-induced priming of human blood leukocytes
Author(s): Tatyana V. Chichuk; Eugeny Ph. Stranadko; I. A. Strashkevich; Gennady I. Klebanov
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Paper Abstract

We investigated the influence of He-Ne ((lambda) equals 632.8 nm) laser irradiation (LI) on a functional activity of human blood leucocytes. The method of luminol-dependent chemiluminescence with the zymosan-activated phagocytes was used. The leucocytes were irradiated without and in the presence of autologic human blood plasma, containing of the endogenous (porphyrins) and/or exogenous (phthalocyanine) photosensitizers. The LI initiated a priming of the leucocytes. Priming revealed itself after the activation of the phagocytes by zymosan. The changes of the calcium concentration in leucocytes cytoplasm were studied too. Fluorimetric method with Fura-2AM was used for this. The laser irradiation initiated the changes of the calcium concentration in the leucocytes cytoplasm. All the investigating parameters depended on the irradiation dose and on the concentration of photosensitizers. The results of this work allowed to formulate the main theses of the free radical mechanism of the low intensive laser irradiation action on human blood leucocytes.

Paper Details

Date Published: 30 December 1999
PDF: 6 pages
Proc. SPIE 4059, Laser Use in Oncology II, (30 December 1999); doi: 10.1117/12.375254
Show Author Affiliations
Tatyana V. Chichuk, State Research Ctr. for Laser Medicine (Russia)
Eugeny Ph. Stranadko, State Research Ctr. for Laser Medicine (Russia)
I. A. Strashkevich, Russian State Medical Univ. (Russia)
Gennady I. Klebanov, Russian State Medical Univ. (Russia)


Published in SPIE Proceedings Vol. 4059:
Laser Use in Oncology II
Andrei V. Ivanov; Mishik A. Kazaryan, Editor(s)

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