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Proceedings Paper

Combinatorial methods: aptamers and aptazymes
Author(s): Andrew D. Ellington; Jay Hesselberth; Sulay Jhaveri; Michael P. Robertson
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Paper Abstract

Combinatorial methods have been used to generate nucleic acid molecules with specific characteristics. Aptamers are nucleic acid binding species, and can be modified to directly transduce molecular recognition to optical signals. Aptazymes are allosteric or effector-activated ribyzymes. We have designed or selected aptazymes that are responsive to a variety of ligands. In particular, we have selected a ribozyme ligase that is activated 10,000-fold in the presence of an oligonucleotide effector, and have designed ligases that are up to 1,600-fold dependent on small molecule effectors. Even in those instances where designed constructs were initially unresponsive, we have been able to use selection to optimize their response characteristics.

Paper Details

Date Published: 15 December 1999
PDF: 9 pages
Proc. SPIE 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection, (15 December 1999); doi: 10.1117/12.372908
Show Author Affiliations
Andrew D. Ellington, Univ. of Texas/Austin (United States)
Jay Hesselberth, Univ. of Texas/Austin (United States)
Sulay Jhaveri, Univ. of Texas/Austin (United States)
Michael P. Robertson, Univ. of Texas/Austin (United States)


Published in SPIE Proceedings Vol. 3858:
Advanced Materials and Optical Systems for Chemical and Biological Detection
Mahmoud Fallahi; Basil I. Swanson, Editor(s)

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