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Proceedings Paper

Integrated optical toxin sensor
Author(s): Dan Kelly; Xuedong Song; Daniel Keith Frayer; Sergio Brito Mendes; Nasser Peyghambarian; Basil I. Swanson; Karen M. Grace
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Paper Abstract

We have developed a method for simple and highly sensitive detection of multivalent proteins using an optical waveguide sensor. The optical biosensor is based on optically tagged glycolipid receptors imbedded within a fluid phospholipid bilayer membrane formed on the surface of a planar optical waveguide. The binding of multivalent toxin initiates a fluorescence resonance energy transfer resulting in a distinctive spectral signature that is monitored by measuring emitted luminescence above the waveguide surface. The sensor methodology is highly sensitive and specific, and requires no additional reagents or washing steps. Demonstration of the utility of protein-receptor recognition using planar optical waveguides is shown here by the detection of cholera toxin.

Paper Details

Date Published: 15 December 1999
PDF: 5 pages
Proc. SPIE 3858, Advanced Materials and Optical Systems for Chemical and Biological Detection, (15 December 1999); doi: 10.1117/12.372899
Show Author Affiliations
Dan Kelly, Los Alamos National Lab. (United States)
Xuedong Song, Los Alamos National Lab. (United States)
Daniel Keith Frayer, Optical Sciences Ctr./Univ. of Arizona (United States)
Sergio Brito Mendes, Optical Sciences Ctr./Univ. of Arizona (United States)
Nasser Peyghambarian, Optical Sciences Ctr./Univ. of Arizona (United States)
Basil I. Swanson, Los Alamos National Lab. (United States)
Karen M. Grace, Los Alamos National Lab. (United States)


Published in SPIE Proceedings Vol. 3858:
Advanced Materials and Optical Systems for Chemical and Biological Detection
Mahmoud Fallahi; Basil I. Swanson, Editor(s)

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