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Proceedings Paper

Optical detection of polychlorinated biphenyls
Author(s): Gabriela Kuncova; Daniela Berkova; Jiri Burkhard; Katerina Demnerova; Jarmila Pazlarova; Jan Triska; Nadezda Vrchotova
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Paper Abstract

In this paper we describe the detection of polychlorinated biphenyls (PCBs) which is based on the measurement of changes of optical absorption at 400 nm of the medium in an aerobic bioreactor with immobilized cells Pseudomonas species 2. The rate of production, composition and the concentration of yellow intermediates are influenced by concentration and composition of PCB mixtures, concentration of cells and by the methods of immobilization. The method was applied in the detection of commercial mixture D103. It was found that the advantageous carriers were inorganic or organic-inorganic matrices, which sorbed PCBs and a cell outgrowth from their surface was low. In water contaminated with transformer oil and chlorinated hydrocarbons the detection limit is 10-2 gD103/kg. In transformer oil the upper limit for degradation of D103 by sodium dehalogenation (1.5 gD103 /kgoil) was determined also in the presence of the same concentration of trichloroethylene. The employment to of a liquid core waveguide spectrophotometer instead of a diode array spectrophotometer increased the sensitivity of the measurement of yellow intermediates by a factor of 100. An extrinsic fiber-optic sensor was used for in-situ measurement during biodegradation of PCBs in bioreactors.

Paper Details

Date Published: 21 December 1999
PDF: 9 pages
Proc. SPIE 3853, Environmental Monitoring and Remediation Technologies II, (21 December 1999); doi: 10.1117/12.372879
Show Author Affiliations
Gabriela Kuncova, Institute of Chemical Process Fundamentals (Czech Republic)
Daniela Berkova, Institute of Radio Engineering and Electronics (Czech Republic)
Jiri Burkhard, Institute of Chemical Technology (Czech Republic)
Katerina Demnerova, Institute of Chemical Technology (Czech Republic)
Jarmila Pazlarova, Institute of Chemical Technology (Czech Republic)
Jan Triska, Institute of Landscape Ecology (Czech Republic)
Nadezda Vrchotova, Institute of Landscape Ecology (Czech Republic)


Published in SPIE Proceedings Vol. 3853:
Environmental Monitoring and Remediation Technologies II
Tuan Vo-Dinh; Robert L. Spellicy, Editor(s)

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