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Proceedings Paper

Real-time detection and characterization of individual flowing airborne biological particles: fluorescence spectra and elastic scattering measurements
Author(s): Yongle Pan; Stephen Holler; Richard K. Chang; Steven C. Hill; Ronald G. Pinnick; Stanley Niles; Jerold R. Bottiger; Burt V. Bronk
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Paper Abstract

Real-time methods which is reagentless and could detect and partially characterize bioaerosols are of current interest. We present a technique for real-time measurement of UV-excited fluorescence spectra and two-dimensional angular optical scattering (TAOS) from individual flowing biological aerosol particles. The fluorescence spectra have been observed from more than 20 samples including Bacillus subtilis, Escherichia coli, Erwinia herbicola, allergens, dust, and smoke. The S/N and resolution of the spectra are sufficient for observing small lineshape differences among the same type of bioaerosol prepared under different conditions. The additional information from TAOS regarding particle size, shape, and granularity has the potential of aiding in distinguishing bacterial aerosols from other aerosols, such as diesel and cigarette smoke.

Paper Details

Date Published: 30 November 1999
PDF: 9 pages
Proc. SPIE 3855, Air Monitoring and Detection of Chemical and Biological Agents II, (30 November 1999); doi: 10.1117/12.371270
Show Author Affiliations
Yongle Pan, New Mexico State Univ. (United States)
Stephen Holler, Yale Univ. (United States)
Richard K. Chang, Yale Univ. (United States)
Steven C. Hill, U.S. Army Research Lab. (United States)
Ronald G. Pinnick, U.S. Army Research Lab. (United States)
Stanley Niles, U.S. Army Research Lab. (United States)
Jerold R. Bottiger, U.S. Army Soldier and Biological Chemical Command (United States)
Burt V. Bronk, U.S. Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 3855:
Air Monitoring and Detection of Chemical and Biological Agents II
Joseph Leonelli; Mark L.G. Althouse, Editor(s)

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