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Proceedings Paper

Airborne particle characterization by spatial scattering and fluorescence
Author(s): John Barton; Edwin Hirst; Paul Kaye; Spencer Saunders; Don Clark
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Paper Abstract

Several workers have reported the development of systems which allow the measurement of intrinsic fluorescence from particles irradiated with ultra-violet radiation. The fluorescence data are frequently recorded in conjunction with other parameters such as particle size, measured either as a function of optical scatter or as an aerodynamic size. The motivation for this work has been principally the detection of bioaerosols within an ambient environment. Previous work by the authors has shown that an analysis of the scattering profile of a particle, i.e.: the spatial distribution of light scattered by the particle carried in a sample air-stream, can provide an effective means of particle characterization and classification in terms of both size and shape parameters. Current work is aimed at the simultaneous recording of both spatial scattering and fluorescence data from individual particles with a view to substantially enhanced discrimination of biological aerosols. A prototype instrument has recently been completed which employs a cw 266 nm laser source to produce both elastic (spatial scattering) and inelastic (fluorescence) signals from individual airborne particles. The instrument incorporates a custom designed high-gain multi- pixel hybrid photodiode (HPD) to record the spatial scattering data and a single photomultiplier to record total fluorescence from the illuminated particle. Recorded data are processed to allow the classification of airborne particles on the basis of size, shape, and fluorescence for both biological and non- biological aerosols.

Paper Details

Date Published: 30 November 1999
PDF: 9 pages
Proc. SPIE 3855, Air Monitoring and Detection of Chemical and Biological Agents II, (30 November 1999); doi: 10.1117/12.371267
Show Author Affiliations
John Barton, Univ. of Hertfordshire (United Kingdom)
Edwin Hirst, Univ. of Hertfordshire (United Kingdom)
Paul Kaye, Univ. of Hertfordshire (United Kingdom)
Spencer Saunders, Univ. of Hertfordshire (United Kingdom)
Don Clark, Defense Evaluation and Research Agency Porton Down (United Kingdom)


Published in SPIE Proceedings Vol. 3855:
Air Monitoring and Detection of Chemical and Biological Agents II
Joseph Leonelli; Mark L.G. Althouse, Editor(s)

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