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Proceedings Paper

Structural elements and fluctuations due to phase separation in adult bovine lenses
Author(s): Bronislaw Grzegorzewski; Volodymyr M. Rudeychuk
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Paper Abstract

The nuclear part of adult bovine lens is investigated by means of static light scattering. The analysis of the angular intensity distribution of the scattered light permits to separate two temperature regions where different properties of the scattered light are observed. The hypothesis about the existence of binary liquid phase separation in the cytoplasm of the adult bovine lens is proposed. Accordingly, in the stable state of the cytoplasm the scattering occurs via structure elements of the lens only and the random density fluctuation theory is adopted to evaluate the parameters of the elements. On approach of the spinodal by lowering the temperature fluctuations characteristic for critical ones are deduced. The Orstein- Zernike theory adopted for analysis of these fluctuations shows that the temperature dependence of the respective correlation length is similar to the dependence for binary liquid phase separation. The spinodal temperature is estimated. In the temperature region below the spinodal protein aggregates and spheres polydisperse in size give rise to the scattering.

Paper Details

Date Published: 18 November 1999
PDF: 9 pages
Proc. SPIE 3904, Fourth International Conference on Correlation Optics, (18 November 1999); doi: 10.1117/12.370445
Show Author Affiliations
Bronislaw Grzegorzewski, Ludwik Rydygier Medical Univ. (Poland)
Volodymyr M. Rudeychuk, Chernivtsy State Univ. (Ukraine)


Published in SPIE Proceedings Vol. 3904:
Fourth International Conference on Correlation Optics
Oleg V. Angelsky, Editor(s)

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