Share Email Print
cover

Proceedings Paper

Infuence of fiber terminal face reflection on fiber optical confocal scanning microscope
Author(s): Lisong Yang; Guiying Wang; Jiangang Wang; Zhizhan Xu
Format Member Price Non-Member Price
PDF $17.00 $21.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In fiber optical confocal scanning imaging system of reflection mode, interference of the reflection light beams from terminal face of the fiber coupling tip and from the surface of the sample will always destroy the image of low reflection index samples, such as CD-R pregroove basal disc and biotic samples. A quantitative analysis was given to find out the influence of fiber terminal face reflection on the system axial response. In order to avoid the influence of the fiber reflected light, the reflectivity ratio of the fiber tip to the sample should be low. Interference noise was effectively reduced by immersing the unused fiber tip into glycerol and cleaving the fiber tip end face at an angle. The proof experimental results of axial response have been shown. Finally, good quality confocal images of the recordable CD pregroove and the chromosome were presented.

Paper Details

Date Published: 17 September 1999
PDF: 5 pages
Proc. SPIE 3863, 1999 International Conference on Biomedical Optics, (17 September 1999); doi: 10.1117/12.364408
Show Author Affiliations
Lisong Yang, Shanghai Institute of Optics and Fine Mechanics (Ireland)
Guiying Wang, Shanghai Institute of Optics and Fine Mechanics (China)
Jiangang Wang, Shanghai Institute of Optics and Fine Mechanics (China)
Zhizhan Xu, Shanghai Institute of Optics and Fine Mechanics (China)


Published in SPIE Proceedings Vol. 3863:
1999 International Conference on Biomedical Optics
Qingming Luo; Britton Chance; Lihong V. Wang; Steven L. Jacques, Editor(s)

© SPIE. Terms of Use
Back to Top