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Proceedings Paper

Laser micromachined isoelectric focusing devices on polymer substrate for electrospray mass spectrometry
Author(s): Yuehe Lin; Jenny Wen; Xiang Fan; Dean W. Matson; Richard D. Smith
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Paper Abstract

A microfabricated device for isoelectric focusing (IEF) incorporating an optimized electrospray ionization (ESI) tip was constructed on polycarbonate plates using a laser micromachining technique. The separation channels on an IEF chip were 16 cm long, 50 micrometers wide and 30 micrometers deep. Electrical potentials used for IEF focusing and electrospray were applied through platinum electrodes placed in the buffer reservoirs, and which were isolated from the separation channel by molecular porous membranes. On-line ESI produced directly from a sharp `tip' on the microchip was evaluated. The results indicate that this design can produce a stable electrospray that is further improved and made more flexible with the assistance of sheath gas and sheath liquid. Error analysis of the spectral data shows that the standard deviation in signal intensity for an analyte peak was less than approximately 5% over 3 hours. The production of stable electrosprays directly from microchip IEF devices represents a step towards easily- fabricated microanalytical devices. IEF separations of protein mixtures were demonstrated for uncoated polycarbonate microchips. On-line IEF/ESI-MS was demonstrated using the microfabricated chip with an ion-trap ESI mass spectrometer for characterization of protein mixtures.

Paper Details

Date Published: 19 August 1999
PDF: 8 pages
Proc. SPIE 3877, Microfluidic Devices and Systems II, (19 August 1999); doi: 10.1117/12.359351
Show Author Affiliations
Yuehe Lin, Pacific Northwest National Lab. (United States)
Jenny Wen, Pacific Northwest National Lab. (United States)
Xiang Fan, Pacific Northwest National Lab. (United States)
Dean W. Matson, Pacific Northwest National Lab. (United States)
Richard D. Smith, Pacific Northwest National Lab. (United States)

Published in SPIE Proceedings Vol. 3877:
Microfluidic Devices and Systems II
Chong Hyuk Ahn; A. Bruno Frazier, Editor(s)

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