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Proceedings Paper

Multiwavelength videomicrofluorimetric method for a preliminary, fast, and inexpensive screening of the cytoxic properties of new drugs: application to some new marine peptides
Author(s): Pierre M. Viallet; Emmanuelle Rocchi; Jean Vigo; Jean-Marie Salmon
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Paper Abstract

The discovery of new families of active drugs isolated from natural sources is an appealing avenue to avoid the MDR consequences in cancer treatment. Unfortunately the procedures of isolation and purification of these compounds are generally complex and time-consuming so that only tiny amounts of the new drugs are available for the preliminary biological tests. As a consequence there is a need for reliable, quantitative methods, more informative than the conventional cytotoxicity curve on the potential intracellular targets, and for which only tiny amounts of drugs are necessary. We have recently developed a method of screening based on a triple labeling process of single living cells in culture, involving the simultaneous use of Hoechst 33342 for nuclear staining. Rhodamine 123 for mitochondrial staining and Nile Red for cell delineation. Numerical image analysis was used to study both the dose- effect and the time-effect of well-known cytotoxic drugs such adriamycin, allowing to select the more informative biochemical parameters. The use of this method has recently been extended to new marine peptides such as didemnin B and some laxaphycins.

Paper Details

Date Published: 9 July 1999
PDF: 11 pages
Proc. SPIE 3595, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems, (9 July 1999); doi: 10.1117/12.351540
Show Author Affiliations
Pierre M. Viallet, Univ. de Perpignan (France)
Emmanuelle Rocchi, Univ. de Perpignan (France)
Jean Vigo, Univ. de Perpignan (France)
Jean-Marie Salmon, Univ. de Perpignan (France)

Published in SPIE Proceedings Vol. 3595:
Biomedical Diagnostic, Guidance, and Surgical-Assist Systems
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Steven T. Charles; Richard D. Bucholz; Michael W. Vannier, Editor(s)

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