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Proceedings Paper

Mag-Indo-1 protein interaction as a tool for probing the 3D conformation of protein subdomains: influence of the chemical microenvironment of the histidin residue(s) on the paramaters of the intera
Author(s): Pierre M. Viallet; Tuan Vo-Dinh; Terry Bunde; Anne-Cecile Ribou; Jean Vigo; Jean-Marie Salmon
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Paper Abstract

Magnesium complexation with Mag-indo-1 results in a shift of the emission fluorescence spectrum from 480 nm to 417 nm. Mag-indo-1 is also able to bind calcium and zinc. These cationic interactions induce the same spectral shift but the fluorescence intensity and the dissociation constant are dependent of the nature of the cation. Furthermore Mag-indo- 1 bind also proteins through a specific interaction with some histidin residues. That interaction induces a characteristic spectral shift of the emission fluorescence spectra from 480 to 457 nM.

Paper Details

Date Published: 9 July 1999
PDF: 12 pages
Proc. SPIE 3595, Biomedical Diagnostic, Guidance, and Surgical-Assist Systems, (9 July 1999); doi: 10.1117/12.351536
Show Author Affiliations
Pierre M. Viallet, Univ. de Perpignan (France)
Tuan Vo-Dinh, Oak Ridge National Lab. (United States)
Terry Bunde, Oak Ridge National Lab. (United States)
Anne-Cecile Ribou, Univ. de Perpignan (France)
Jean Vigo, Univ. de Perpignan (France)
Jean-Marie Salmon, Univ. de Perpignan (France)

Published in SPIE Proceedings Vol. 3595:
Biomedical Diagnostic, Guidance, and Surgical-Assist Systems
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Steven T. Charles; Richard D. Bucholz; Michael W. Vannier, Editor(s)

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