Share Email Print
cover

Proceedings Paper

Distribution of protoporphyrin IX (PPIX) induced by aminolevulinic acid (5-ALA) in the skin and liver of rats
Author(s): Sergio Kaneo Furuzawa; Renato Amaro Zangaro; Renata Amadei Nicolau; Marcos T. T. Pacheco
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Photodynamic diagnosis by fluorescence emission from PPIX induced by 5-ALA can be done with minimal damage to cells using excitation by low power blue light. This technique uses the fluorescence signal emitted by the PPIX to obtain information about its concentration and dynamics without cell damage. The intracellular peak concentration of PPIX has a distinct time dependence for each organ. We have measured the accumulation time for maximum PPIX concentration in Wister rats (liver and skin) detecting the induced fluorescence in each organ. PPIX accumulation time varies depending on the tissue type. The liver shows a higher PPIX concentration. The detection system can measure endogenous PPIX. This fact opens the possibility of in-vivo displastic tissue diagnosis since these tissues have significant endogenous PPIX concentration.

Paper Details

Date Published: 6 July 1999
PDF: 5 pages
Proc. SPIE 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII, (6 July 1999); doi: 10.1117/12.351510
Show Author Affiliations
Sergio Kaneo Furuzawa, Univ. do Vale do Paraiba (Brazil)
Renato Amaro Zangaro, Univ. do Vale do Paraiba (Brazil)
Renata Amadei Nicolau, Univ. do Vale do Paraiba (Brazil)
Marcos T. T. Pacheco, Univ. do Vale do Paraiba (Brazil)


Published in SPIE Proceedings Vol. 3592:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII
Thomas J. Dougherty, Editor(s)

© SPIE. Terms of Use
Back to Top