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Proceedings Paper

Fluorescent imaging of cells and nucleic acids in bioelectronic chips
Author(s): Jing Cheng; Lei Wu; Jonathan Diver; Paul N. Swanson; Adam Uribe; Michael Krihak; Jainamma Krotz; Kenneth A. Bloch; Bill Street; Trevor Young; Tony Llevares; Tim LeClair; Donald E. Ackley; Michael J. Heller; James P. O'Connell; Tina S. Nova
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Paper Abstract

We have performed separation of bacterial and cultured cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also related DNA and RNA from the isolated cells electrically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts toward the construction of a 'laboratory-on- a-chip' system will be presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Paper Details

Date Published: 2 July 1999
PDF: 6 pages
Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351041
Show Author Affiliations
Jing Cheng, Nanogen, Inc. (United States)
Lei Wu, Nanogen, Inc. (United States)
Jonathan Diver, Nanogen, Inc. (United States)
Paul N. Swanson, Nanogen, Inc. (United States)
Adam Uribe, Nanogen, Inc. (United States)
Michael Krihak, Nanogen, Inc. (United States)
Jainamma Krotz, Nanogen, Inc. (United States)
Kenneth A. Bloch, Nanogen, Inc. (United States)
Bill Street, Nanogen, Inc. (United States)
Trevor Young, Nanogen, Inc. (United States)
Tony Llevares, Nanogen, Inc. (United States)
Tim LeClair, Nanogen, Inc. (United States)
Donald E. Ackley, Nanogen, Inc. (United States)
Michael J. Heller, Nanogen, Inc. (United States)
James P. O'Connell, Nanogen, Inc. (United States)
Tina S. Nova, Nanogen, Inc. (United States)


Published in SPIE Proceedings Vol. 3600:
Biomedical Imaging: Reporters, Dyes, and Instrumentation
Eva Marie Sevick-Muraca; Darryl J. Bornhop; Christopher H. Contag; Eva Marie Sevick-Muraca, Editor(s)

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