Share Email Print
cover

Proceedings Paper

Fluorescence lifetime measurements of NADH and tryptophan in intact ischemic, intact rabbit myocardium
Author(s): Adrian Hamburger; Zygmunt Gryczynski; Joseph R. Lakowicz; Keith Sommers
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Ischemia-reperfusion injury is the leading cause of early dysfunction following transplantation. Currently, there are no techniques available to accurately measure ischemic changes during organ storage. Therefore, the interest exists in developing non-invasive monitoring techniques. We used NADH and tryptophan as fluorescent markers, since both are intrinsic fluorophores and excellent indicators for levels of hypoxia and protein denaturation, respectively.

Paper Details

Date Published: 2 July 1999
PDF: 6 pages
Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351040
Show Author Affiliations
Adrian Hamburger, Univ. of South Florida College of Medicine (United States)
Zygmunt Gryczynski, Univ. of Maryland/Baltimore School of Medicine (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore School of Medicine (United States)
Keith Sommers, Univ. of South Florida College of Medicine (United States)


Published in SPIE Proceedings Vol. 3600:
Biomedical Imaging: Reporters, Dyes, and Instrumentation
Eva Marie Sevick-Muraca; Darryl J. Bornhop; Christopher H. Contag; Eva Marie Sevick-Muraca, Editor(s)

© SPIE. Terms of Use
Back to Top