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Proceedings Paper

Microscope-based single photon imaging for studying the functional dynamics of living cells using luminesent markers
Author(s): Eric Karplus; Anastasia Kanellopoulos
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Paper Abstract

A microscope-based imaging system has been developed that records single photon emissions from luminescent samples. The time and position of each detected photon is recorded so that integration times for image reconstruction can be selected and adjusted during data analysis. The system performs automated acquisition of brightfield and epifluorescence images that are correlated with the photon images. The features of this system will be demonstrated in the context of studying the dynamics of living cells using luminescent markers such as luciferase and aequorin, which can be introduced by microinjection or transfection. The value of using this approach to study the effects of pharmacological agents on gene expression in living cells will be presented.

Paper Details

Date Published: 2 July 1999
PDF: 11 pages
Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351034
Show Author Affiliations
Eric Karplus, Science Wares (United States)
Anastasia Kanellopoulos, Science Wares (United States)

Published in SPIE Proceedings Vol. 3600:
Biomedical Imaging: Reporters, Dyes, and Instrumentation
Eva Marie Sevick-Muraca; Darryl J. Bornhop; Christopher H. Contag; Eva Marie Sevick-Muraca, Editor(s)

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