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Proceedings Paper

Time-resolved laser-induced fluorescence of normal and atherosclerotic coronary artery
Author(s): Laura Marcu; Jean-Michel I. Maarek; Michael C. Fishbein; Warren S. Grundfest
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Paper Abstract

This study investigates the spectro-temporal fluorescence emission of normal and diseased coronary arteries with graded levels of atherosclerosis. Fluorescence emission of 58 excised human coronary artery samples was induced with N2 laser pulses and detected with a MCP-PMT connected to a digital oscilloscope. The samples were H and E and Movat stained and histologically classified in accordance with AHA classification. An algorithm based on Laguerre expansion of kernels was used to deconvolve the intrinsic fluorescence impulse response function from the measured transient pulse. A biexponential function depicted the fluorescence decay characteristics. We noticed 1) in spectral domain: peak fluorescence intensity was at 380 nm for normal and initial lesions samples and blue-shifted for advanced lesions; intensity at 450-480 nm decreased from approximately 65 percent peak intensity for normal samples to approximately 30 percent for Type V lesions; 2) in time domain: longer lasting emission for the advanced lesions. The decay constants varied as a function emission wavelength and lesion type. For instance the time constants for Type V lesions measured at 390 nm were significantly larger that those measured on normal arterial wall. The fast term decay contributed to a higher degree to the impulse response function for normal tissue. These results reveal that the analysis of the temporal characteristics of fluorescence can be used to differentiate between coronary lesion and normal coronary wall. The time domain information complements the spectral domain intensity data for improved differentiation between graded levels of coronary lesions.

Paper Details

Date Published: 2 July 1999
PDF: 10 pages
Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351029
Show Author Affiliations
Laura Marcu, Univ. of Southern California and Cedars-Sinai Medical Ctr. (United States)
Jean-Michel I. Maarek, Univ. of Southern California (United States)
Michael C. Fishbein, Cedars-Sinai Medical Ctr. and Univ. of California/Los Angeles School of Medicine (United States)
Warren S. Grundfest, Univ. of Southern California and Cedars-Sinai Medical Ctr. and UCLA School of Medicine (United States)

Published in SPIE Proceedings Vol. 3600:
Biomedical Imaging: Reporters, Dyes, and Instrumentation
Eva Marie Sevick-Muraca; Darryl J. Bornhop; Christopher H. Contag; Eva Marie Sevick-Muraca, Editor(s)

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