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Proceedings Paper

Time-resolved in-situ measurement of mitochondrial malfunction by energy transfer spectroscopy
Author(s): Herbert Schneckenburger; Michael H. Gschwend; Wolfgang S. L. Strauss; Reinhard Sailer; Lars Schoch; Alexander Schuh; Karl Stock; Rudolf W. Steiner; Peter Zipfl
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Paper Abstract

To establish optical in situ detection of mitochondrial malfunction, non-radiative energy transfer from the coenzyme NADH to the mitochondrial marker rhodamine 123 (R123) was examined. Dual excitation of R123 via energy transfer from excited NADH molecules as well as by direct absorption of light results in two fluorescence signals whose ratio is a measure of mitochondrial NADH. An experimental setup was developed, where these signals are detected simultaneously using a time-gated technique for energy transfer measurements and a frequency selective technique for direct excitation and fluorescence monitoring of R123. Optical and electronic components of the apparatus are described, and preliminary result of cultivated endothelial cells are reported. Results are compared with those obtained from a previously established microscopic system and discussed in view of potential applications.

Paper Details

Date Published: 2 July 1999
PDF: 6 pages
Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351027
Show Author Affiliations
Herbert Schneckenburger, Univ. Ulm and Fachhochschule Aalen (Germany)
Michael H. Gschwend, Institut fuer Lasertechnologien in der Medizin und Messtechnik an der Univ. Ulm (Germany)
Wolfgang S. L. Strauss, Institut fuer Lasertechnologien in der Medizin und Messtechnik an der Univ. Ulm (Germany)
Reinhard Sailer, Institut fuer Lasertechnologien in der Medizin und Messtechnik an der Univ. Ulm (Germany)
Lars Schoch, Fachhochschule Aalen (Germany)
Alexander Schuh, Fachhochschule Aalen (Germany)
Karl Stock, Univ. Ulm and Fachhochschule Aalen (Germany)
Rudolf W. Steiner, Institut fuer Lasertechnologien in der Medizin und Messtechnik an der Univ. Ulm (Germany)
Peter Zipfl, Fachhochschule Aalen (Germany)


Published in SPIE Proceedings Vol. 3600:
Biomedical Imaging: Reporters, Dyes, and Instrumentation
Eva Marie Sevick-Muraca; Darryl J. Bornhop; Christopher H. Contag; Eva Marie Sevick-Muraca, Editor(s)

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