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Proceedings Paper

Transferrin-mediated tumor delivery of contrast media for optical imaging and magnetic resonance imaging
Author(s): Andreas Becker; Kai Licha; Mayk Kresse; Bjoern Riefke; Uwe Sukowski; Bernd Ebert; Herbert H. Rinneberg; Wolfhard Semmler
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Paper Abstract

Many tumor cells are characterized by the overexpression of highly specific transferrin receptors. Transferrin is bound to its receptor, which is followed by rapid internalization into intracellular compartments; it can thus be used for the specific delivery of coupled contrast agents in to tumor cells. The aim of our study was to investigate the tumor- specificity of transferrin-modified contrast agents for optical imaging and magnetic resonance imaging (MRI). Indotricarbocyanine, a near-IR absorbing dye, and ultrasmall superparamagnetic iron oxide particles, a contrast material for MRI, were covalently coupled to transferrin and examined in vitro and in vivo for their potential as tumor-specific contrast agents. CEll culture experiments using HT29 and A431 tumor cells showed specific uptake of transferrin- modified contrast agents by the cells at 37 degrees C, while uptake was inhibited at 4 degrees C or in the presence of an excess of unlabeled transferrin. Optical imaging of tumor- bearing nude mice after intravenous injection of transferrin-ICC resulted in pronounced tumor fluorescence. Tumor specificity was also observe in MRI, where transferrin-USPIO accumulated in SMT2A tumor-bearing rats. We conclude that transferrin-modified contrast agents for optical imaging and MRI can be specifically delivered to tumor tissue by use of transferrin receptor pathways.

Paper Details

Date Published: 2 July 1999
PDF: 9 pages
Proc. SPIE 3600, Biomedical Imaging: Reporters, Dyes, and Instrumentation, (2 July 1999); doi: 10.1117/12.351024
Show Author Affiliations
Andreas Becker, Institut fuer Diagnostikforschung an der Freien Univ. Berlin (Germany)
Kai Licha, Institut fuer Diagnostikforschung and der Freien Univ. Berlin (Germany)
Mayk Kresse, Institut fuer Diagnostikforschung an der Freien Univ. Berlin (Germany)
Bjoern Riefke, Institut fuer Diagnostikforschung an der Freien Univ. Berlin (Germany)
Uwe Sukowski, Physikalisch-Technische Bundesanstalt (Germany)
Bernd Ebert, Physikalisch-Technische Bundesanstalt (Germany)
Herbert H. Rinneberg, Physikalisch-Technische Bundesanstalt (Germany)
Wolfhard Semmler, Institut fuer Diagnostikforschung an der Freien Univ. Berlin (Germany)


Published in SPIE Proceedings Vol. 3600:
Biomedical Imaging: Reporters, Dyes, and Instrumentation
Eva Marie Sevick-Muraca; Darryl J. Bornhop; Christopher H. Contag; Eva Marie Sevick-Muraca, Editor(s)

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