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Proceedings Paper

Scanning near-field optical/atomic-force microscope (SNOAM) for biomedical applications
Author(s): Eiichi Tamiya; Shinichiro Iwabuchi; Atsuko Hashigasako; Yuji Murakami; Toshifumi Sakaguchi; Yasutaka Morita; Kenji Yokoyama
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Paper Abstract

A scanning near-field optical/atomic-force microscope (SNOAM) system was applied to simultaneous topographic and fluorescence imaging of biological samples in air and liquid. The SNOAM uses a bent optical fiber simultaneously as a dynamic mode atomic-force microscopy cantilever and a scanning near-field optical microscopy probe. The SNOAM system used 458 or 488 nm from Ar ion laser multiline of excitation of green fluorescent protein (GFP), since a native GFP has been known to give a maximum at 395 nm and a broad absorption spectrum until 500 nm. Topographic and fluorescence images of recombinant E.coli were obtained simultaneously with a high spatial resolution which was apparently better than that of a conventional confocal microscope.

Paper Details

Date Published: 17 June 1999
PDF: 10 pages
Proc. SPIE 3607, Scanning and Force Microscopies for Biomedical Applications, (17 June 1999); doi: 10.1117/12.350635
Show Author Affiliations
Eiichi Tamiya, Japan Advanced Institute of Science and Technology (Japan)
Shinichiro Iwabuchi, Japan Advanced Institute of Science and Technology (Japan)
Atsuko Hashigasako, Japan Advanced Institute of Science and Technology (Japan)
Yuji Murakami, Japan Advanced Institute of Science and Technology (Japan)
Toshifumi Sakaguchi, Japan Advanced Institute of Science and Technology (Japan)
Yasutaka Morita, Japan Advanced Institute of Science and Technology (Japan)
Kenji Yokoyama, Japan Advanced Institute of Science and Technology (Japan)


Published in SPIE Proceedings Vol. 3607:
Scanning and Force Microscopies for Biomedical Applications
Eiichi Tamiya; Shuming Nie, Editor(s)

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