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Proceedings Paper

Detecting exposure to environmental organic toxins in individual cells: toward development of a microfabricated device
Author(s): Hoi-Ying N. Holman; Miqin Zhang; Regine Goth-Goldstein; Michael C. Martin; Marion Russell; Wayne R. McKinney; Mauro Ferrari; Jennie C. Hunter-Cevera
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Paper Abstract

A new method is being developed for quickly screen for the human exposure potential to polycyclic aromatic hydrocarbons (PAHs) and organochlorines (OCs). The development involves two key elements: identifying suitable signals that represent intracellular changes that are specific to PAH and OC exposure, and constructing a device to guide the biological cell growth so that signals from individual cells are consistent and reproducible. We are completing the identification of suitable signals by using synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy in the mid-infrared region (4000 - 400 cm-1). Distinct changes have been observed in the IR spectra after treatment of human cells in culture medium with PAHs and OCs. The potential use of this method for detecting exposure to PAHs and OCs has been tested and compared to a reverse transcription polymerase chain reaction (RT-PCR) assay that quantifies increased expression of the CYP1A1 gene in response to exposure to PAHs or OCs.

Paper Details

Date Published: 3 June 1999
PDF: 8 pages
Proc. SPIE 3606, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II, (3 June 1999); doi: 10.1117/12.350062
Show Author Affiliations
Hoi-Ying N. Holman, Lawrence Berkeley National Lab. (United States)
Miqin Zhang, Univ. of California/Berkeley (United States)
Regine Goth-Goldstein, Lawrence Berkeley National Lab. (United States)
Michael C. Martin, Lawrence Berkeley National Lab. (United States)
Marion Russell, Lawrence Berkeley National Lab. (United States)
Wayne R. McKinney, Lawrence Berkeley National Lab. (United States)
Mauro Ferrari, Univ. of California/Berkeley and Ohio State Univ. (United States)
Jennie C. Hunter-Cevera, Lawrence Berkeley National Lab. (United States)


Published in SPIE Proceedings Vol. 3606:
Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II
Mauro Ferrari, Editor(s)

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