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Proceedings Paper

Molecular DNA switches and DNA chips
Author(s): Chandran R. Sabanayagam; Cristin Berkey; Uri Lavi; Charles R. Cantor; Cassandra L. Smith
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Paper Abstract

We present an assay to detect single-nucleotide polymorphisms on a chip using molecular DNA switches and isothermal rolling- circle amplification. The basic principle behind the switch is an allele-specific oligonucleotide circularization, mediated by DNA ligase. A DNA switch is closed when perfect hybridization between the probe oligonucleotide and target DNA allows ligase to covalently circularize the probe. Mismatches around the ligation site prevent probe circularization, resulting in an open switch. DNA polymerase is then used to preferentially amplify the closed switches, via rolling-circle amplification. The stringency of the molecular switches yields 102 - 103 fold discrimination between matched and mismatched sequences.

Paper Details

Date Published: 3 June 1999
PDF: 8 pages
Proc. SPIE 3606, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II, (3 June 1999); doi: 10.1117/12.350049
Show Author Affiliations
Chandran R. Sabanayagam, Boston Univ. (United States)
Cristin Berkey, Boston Univ. (United States)
Uri Lavi, The Volcani Ctr. (Israel)
Charles R. Cantor, Boston Univ. and Sequenom, Inc. (United States)
Cassandra L. Smith, Boston Univ. (United States)


Published in SPIE Proceedings Vol. 3606:
Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications II
Mauro Ferrari, Editor(s)

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