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Proceedings Paper

Microheater as an alternative to lasers for in-vitro fertilization applications
Author(s): Daniel V. Palanker; Igor Turovets; Rima Glazer; Benjamin E. Reubinoff; Dalia Hilman; Aaron Lewis
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Paper Abstract

During the last decade various lasers have been applied to drilling of the micrometer-sized holes in the zona pellucida of oocytes for in-vitro fertilization applications. In this paper we describe an alternative approach to laser instrumentation based on microfabricated device capable of precise drilling of uniform holes in the zona pellucida of oocytes. This device consists of a thin (1 micrometer) film microheater built on the tip of glass capillary with a diameter varying between a few to a few tens of micrometers. Duration of the pulse of heat produced by this microheater determines the spatial confinement of the heat wave in the surrounding liquid medium. We have demonstrated that gradual microdrilling of the zona pellucida can be accomplished using a series of pulses with duration of about 300 microseconds when the microheater was held in contact with the zona pellucida. Pulse energy applied to 20 micrometer tip was about 4 (mu) J. In vitro development and hatching of 127 micromanipulated embryos was compared to 103 non-drilled control embryos. The technique was found to be highly efficient in creating round, uniform, well defined holes with a smooth wall surface, matching the size of the heating source. The architecture of the surrounding zona pellucida was unaffected by the drilling, as demonstrated by scanning electron microscopy. Micromanipulated embryos presented no signs of thermal damage under light microscopy. The rate of blastocyst formation and hatching was similar in the micromanipulated and control groups. Following further testing in animal models, this methodology may be used as a cost- effective alternative to laser-based instrumentation in clinical applications such as assisted hatching and embryo biopsy.

Paper Details

Date Published: 14 June 1999
PDF: 8 pages
Proc. SPIE 3601, Laser-Tissue Interaction X: Photochemical, Photothermal, and Photomechanical, (14 June 1999); doi: 10.1117/12.350026
Show Author Affiliations
Daniel V. Palanker, Hadassah Univ. Hospital (United States)
Igor Turovets, Hadassah Univ. Hospital (Israel)
Rima Glazer, Hadassah Univ. Hospital (Israel)
Benjamin E. Reubinoff, Hadassah Univ. Hospital (Israel)
Dalia Hilman, Hadassah Univ. Hospital (Israel)
Aaron Lewis, Hadassah Univ. Hospital (Israel)

Published in SPIE Proceedings Vol. 3601:
Laser-Tissue Interaction X: Photochemical, Photothermal, and Photomechanical
Steven L. Jacques; David H. Sliney; Gerhard J. Mueller; Gerhard J. Mueller; Andre Roggan; Andre Roggan; David H. Sliney, Editor(s)

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